Actin (32 kDa fragment) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of colchicine treated SH-SY5Y cell lysate showing specific immunolabeling of the ~ 32k cleaved actin fragment (fractin).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Actin (32 KDa fragment).
Immunogen
A synthetic peptide corresponding to C-terminus of 32 kDa actin fragment.
Host
Rabbit
Theoretical MW (kDa)
32
Reactivity
Human, Rat
Specificity
This antibody has been directly tested for reactivity in human and rat. specific to the ~ 32 KDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
No additive
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of colchicine treated SH-SY5Y cell lysate showing specific immunolabeling of the ~ 32k cleaved actin fragment (fractin).Immunohistochemistry
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Publication Reference
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Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury.
Rossiter JP, Anderson LL, Yang F, Cole GM.
Acta Neuropathologica 2002 Jan; 103(1):66.
Application：IHC-P, Human, Human brains.
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Multiple-label immunocytochemistry for the evaluation of nature of cell death in experimental models of neurodegeneration.
Adamec E, Yang F, Cole GM, Nixon RA.
Brain Research. Brain Research Protocols 2001 Jul; 7(3):193.
Application：IF, Rat, Hippocampal neurons.
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Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina.
Chen TA, Yang F, Cole GM, Chan SO.
Brain Research 2001 Jun; 904(1):177.
Application：ICC, Rat, Retina.
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Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury.
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