Aqp2 (phospho S264) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of vasopressin (AVP, lane 2) treated rat kidney lysate and control (Lane 1) showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the Aqp2 (phospho S264) polyclonal antibody (Cat ID # PAB16935).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Aqp2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S264 of rat Aqp2.
Host
Rabbit
Theoretical MW (kDa)
29, 37
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Non-Human Primates, Rat
Specificity
This antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that This antibody will react with canine, bovine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. specific to the ~29k AQP2 protein phosphorylated at Ser264. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)
Storage Instruction
For long term store at -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50 % glycerol.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of vasopressin (AVP, lane 2) treated rat kidney lysate and control (Lane 1) showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the Aqp2 (phospho S264) polyclonal antibody (Cat ID # PAB16935). -
Gene Info — Aqp2
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Publication Reference
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Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin.
Fenton RA, Moeller HB, Hoffert JD, Yu MJ, Nielsen S, Knepper MA.
PNAS 2008 Feb; 105(8):3134.
Application：IEM, IF, IHC, WB-Ti, Mouse, Rat, Mouse, Rat kidney.
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Characterization of two novel missense mutations in the AQP2 gene causing nephrogenic diabetes insipidus.
Iolascon A, Aglio V, Tamma G, D'Apolito M, Addabbo F, Procino G, Simonetti MC, Montini G, Gesualdo L, Debler EW, Svelto M, Valenti G.
Nephron. Physiology 2007 Dec; 105(3):p33.
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Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct.
Hoffert JD, Nielsen J, Yu MJ, Pisitkun T, Schleicher SM, Nielsen S, Knepper MA.
American Journal of Physiology. Renal Physiology 2007 Feb; 292(2):F691.
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Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin.
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