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Last updated: 2017/1/15
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TNF polyclonal antibody

  • Catalog # : PAB16934
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant TNF.
  • Immunogen:
  • Recombinant protein corresponding to 154 amino acids of swine TNF.
  • Host:
  • Rabbit
  • Reactivity:
  • Pig
  • Form:
  • Lyophilized
  • Recommend Usage:
  • ELISA (1:2000-1:2500)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using TNF polyclonal antibody (Cat # PAB16934) shows detection of recombinant Swine TNFa at about 16.9 KDa raised in yeast. The identity of the faint band at 135 KDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with 1 ug/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry
  • ELISA
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