Il17f polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using Il17f polyclonal antibody (Cat # PAB16929) shows detection of a band ~18 KDa in size corresponding to recombinant mouse IL17f.
Recombinant mouse IL17f was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose.
The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Il17f.
Immunogen
Recombinant protein corresponding to full length mouse Il17f.
Host
Rabbit
Reactivity
Mouse, Rat
Form
Lyophilized
Recommend Usage
ELISA (1:10000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using Il17f polyclonal antibody (Cat # PAB16929) shows detection of a band ~18 KDa in size corresponding to recombinant mouse IL17f.
Recombinant mouse IL17f was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose.
The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Il17f
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Publication Reference
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Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase.
Gomez-Rodriguez J, Sahu N, Handon R, Davidson TS, Anderson SM, Kirby MR, August A, Schwartzberg PL.
Immunity 2009 Oct; 31(4):587.
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IL-9 as a mediator of Th17-driven inflammatory disease.
Nowak EC, Weaver CT, Turner H, Begum-Haque S, Becher B, Schreiner B, Coyle AJ, Kasper LH, Noelle RJ.
The Journal of Experimental Medicine 2009 Jul; 206(8):1653.
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Abundant c-Fas-associated death domain-like interleukin-1-converting enzyme inhibitory protein expression determines resistance of T helper 17 cells to activation-induced cell death.
Yu Y, Iclozan C, Yamazaki T, Yang X, Anasetti C, Dong C, Yu XZ.
Blood 2009 May; 114(5):1026.
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Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase.
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