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Last updated: 2017/5/21

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Il17f polyclonal antibody

  • Catalog # : PAB16929
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant Il17f.
  • Immunogen:
  • Recombinant protein corresponding to full length mouse Il17f.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse, Rat
  • Form:
  • Lyophilized
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using Il17f polyclonal antibody (Cat # PAB16929) shows detection of a band ~18 KDa in size corresponding to recombinant mouse IL17f.
    Recombinant mouse IL17f was loaded on to an SDS-PAGE gel at 0.25 ug and after separation was transferred to nitrocellulose.
    The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in 1% BSA in TBST overnight at 4°. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Il17f
  • Gene Alias:
  • C87042,IL-17F
  • Gene Description:
  • interleukin 17F
  • Other Designations:
  • -
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