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Last updated: 2021/4/11
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Il1b polyclonal antibody 

  • Catalog # : PAB16928
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant Il1b.
  • Immunogen:
  • Recombinant protein corresponding to mouse Il1b.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse, Rat
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Flow Cytometry
    ELISA (1:1000-5000)
    Fluorescence Microscopy Experiment (1:50-250)
    Immunohistochemistry (1:50-250)
    Immunoprecipitation (1:200-800)
    Neutralization
    Western Blot (1:500-2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse Il1b containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW Il1b precursor molecule. Denatured Il1b will have a 18 kDa band.
  • Neutralization
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of Il1b polyclonal antibody in mouse embryonic kidney tissue.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy after staining of mouse carotid artery tissue with Il1b polyclonal antibody diluted 1:50. Panel (A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel (B) shows anti-Il1b staining of cells after surgical injury of tissue. Panel (C) shows no antibody staining of injured carotid tissue from an Il1b KO mouse.
  • Immunoprecipitation
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Application Image
  • Neutralization
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Gene Information
  • Gene Name:
  • Il1b
  • Gene Alias:
  • IL-1beta,Il-1b
  • Gene Description:
  • interleukin 1 beta
  • Other Designations:
  • OTTMUSP00000016525,interleukin 1, beta
  • Interactome
  • Interactome
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