Csf2 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using Csf2 polyclonal antibody (Cat # PAB16927) shows detection of a band ~14 KDa in size corresponding to recombinant mouse Csf2. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Csf2.
Immunogen
Recombinant protein corresponding to full length mouse Csf2.
Host
Rabbit
Reactivity
Mouse
Form
Lyophilized
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using Csf2 polyclonal antibody (Cat # PAB16927) shows detection of a band ~14 KDa in size corresponding to recombinant mouse Csf2. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — Csf2
Entrez GeneID
12981Gene Name
Csf2
Gene Alias
Csfgm, Gm-CSf, MGC151255, MGC151257, MGI-IGM
Gene Description
colony stimulating factor 2 (granulocyte-macrophage)
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000005830|colony stimulating factor 2|granulocyte-macrophage colony stimulating factor 2|put. GM-CSF
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Publication Reference
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Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes.
Miyatake S, Otsuka T, Yokota T, Lee F, Arai K.
The EMBO Journal 1985 Oct; 4(10):2561.
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The structure and expression of the murine gene encoding granulocyte-macrophage colony stimulating factor: evidence for utilisation of alternative promoters.
Stanley E, Metcalf D, Sobieszczuk P, Gough NM, Dunn AR.
The EMBO Journal 1985 Oct; 4(10):2569.
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Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum.
DeLamarter JF, Mermod JJ, Liang CM, Eliason JF, Thatcher DR.
The EMBO Journal 1985 Oct; 4(10):2575.
Application：ELISA, IP, Neutralization, RIA, Mouse, FDC-P1 cells, Recombinant proteins.
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Structure of the chromosomal gene for granulocyte-macrophage colony stimulating factor: comparison of the mouse and human genes.
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