IL1B polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot showing detection of recombinant human IL1B by IL1B polyclonal antibody (Cat # PAB16926). Recombinant human IL1B was loaded on to an SDS-PAGE gel at 0.1 ug and after separation, transferred to nitrocellulose. The expected band is approximately 18 KDa in size. The membrane was blocked with 1% BSA in TBST for 30min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad Versa Doc® 4000 MP imaging system.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL1B.
Immunogen
Recombinant protein corresponding to human IL1B.
Host
Rabbit
Reactivity
Dog, Human, Primates
Form
Lyophilized
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot showing detection of recombinant human IL1B by IL1B polyclonal antibody (Cat # PAB16926). Recombinant human IL1B was loaded on to an SDS-PAGE gel at 0.1 ug and after separation, transferred to nitrocellulose. The expected band is approximately 18 KDa in size. The membrane was blocked with 1% BSA in TBST for 30min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad Versa Doc® 4000 MP imaging system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL1B
Entrez GeneID
3553Gene Name
IL1B
Gene Alias
IL-1, IL1-BETA, IL1F2
Gene Description
interleukin 1, beta
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq
Other Designations
catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Molecular cloning of the interleukin-1 beta converting enzyme.
Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA, March CJ, Kronheim SR, Druck T, Cannizzaro LA, et al..
Science 1992 Apr; 256(5053):97.
Application：IP, Monkey, COS-7 cells.
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Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA.
PNAS 1984 Dec; 81(24):7907.
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Molecular cloning of the interleukin-1 beta converting enzyme.
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