CCL20 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using CCL20 polyclonal antibody (Cat # PAB16925) shows detection of a band ~26 KDa in size corresponding to recombinant human CCL20 (Lane 1). The identity of thelower molecular weight band is unknown. After transfer, the membrane was blocked overnightwith 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 inblocking buffer for 30 min at RT followed by reaction with Femto Max™ chemiluminescent substrate. Image was captured using Versa Doc™ MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CCL20.
Immunogen
Recombinant protein corresponding to full length human CCL20.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using CCL20 polyclonal antibody (Cat # PAB16925) shows detection of a band ~26 KDa in size corresponding to recombinant human CCL20 (Lane 1). The identity of thelower molecular weight band is unknown. After transfer, the membrane was blocked overnightwith 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 inblocking buffer for 30 min at RT followed by reaction with Femto Max™ chemiluminescent substrate. Image was captured using Versa Doc™ MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCL20
Entrez GeneID
6364Gene Name
CCL20
Gene Alias
CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38
Gene Description
chemokine (C-C motif) ligand 20
Omim ID
601960Gene Ontology
HyperlinkGene Summary
member 20
Other Designations
exodus-1|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A (Cys-Cys), member 20
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Interactome
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Pathway
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Disease
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Publication Reference
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CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups.
Lisignoli G, Manferdini C, Codeluppi K, Piacentini A, Grassi F, Cattini L, Filardo G, Facchini A.
Journal of Cellular Physiology 2009 Oct; 221(1):154.
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Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation.
Punj V, Matta H, Schamus S, Yang T, Chang Y, Chaudhary PM.
Blood 2009 Mar; 113(22):5660.
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C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
Reboldi A, Coisne C, Baumjohann D, Benvenuto F, Bottinelli D, Lira S, Uccelli A, Lanzavecchia A, Engelhardt B, Sallusto F.
Nature Immunology 2009 Mar; 10(5):514.
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CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups.
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