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Last updated: 2017/11/19
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IL33 polyclonal antibody

  • Catalog # : PAB16924
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant IL33.
  • Immunogen:
  • Recombinant protein corresponding to full length human IL33.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Recommend Usage:
  • ELISA (1:20000-1:100000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using IL33 polyclonal antibody (Cat # PAB16924) shows detection of a band ~18 KDa in size corresponding to recombinant human IL33. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • IL33
  • Gene Alias:
  • C9orf26,DKFZp586H0523,DVS27,NF-HEV,NFEHEV,RP11-575C20.2
  • Gene Description:
  • interleukin 33
  • Other Designations:
  • DVS27-related protein,OTTHUMP00000021041,nuclear factor from high endothelial venules
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