IL17A polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using IL17A polyclonal antibody (Cat # PAB16922) shows detection of a band ~17 KDa in size corresponding to recombinant human IL17A. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL17A.
Immunogen
Recombinant protein corresponding to full length human IL17A.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using IL17A polyclonal antibody (Cat # PAB16922) shows detection of a band ~17 KDa in size corresponding to recombinant human IL17A. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL17A
Entrez GeneID
3605Gene Name
IL17A
Gene Alias
CTLA8, IL-17, IL-17A, IL17
Gene Description
interleukin 17A
Omim ID
603149Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq
Other Designations
OTTHUMP00000016597|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
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Interactome
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Pathway
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Disease
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Publication Reference
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The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis.
Paust HJ, Turner JE, Steinmetz OM, Peters A, Heymann F, Holscher C, Wolf G, Kurts C, Mittrucker HW, Stahl RA, Panzer U.
Journal of the American Society of Nephrology 2009 Apr; 20(5):969.
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Interleukin-17A inhibits adipocyte differentiation in human mesenchymal stem cells and regulates pro-inflammatory responses in adipocytes.
Shin JH, Shin DW, Noh M.
Biochemical Pharmacology 2009 Mar; 77(12):1835.
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Hypoxia enhances stimulating effect of amyloid beta peptide (25-35) for interleukin 17 and T helper lymphocyte subtype 17 upregulation in cultured peripheral blood mononuclear cells.
Yin Y, Wen S, Li G, Wang D.
Microbiology and Immunology 2009 Feb; 53(5):281.
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The IL-23/Th17 axis contributes to renal injury in experimental glomerulonephritis.
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