IL17F polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using IL17F polyclonal antibody (Cat # PAB16921) shows detection of a band ~15 kDa in size corresponding to recombinant human IL17F. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL17F.
Immunogen
Recombinant protein corresponding to full length human IL17F.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
ELISA (1:1000-1:5000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using IL17F polyclonal antibody (Cat # PAB16921) shows detection of a band ~15 kDa in size corresponding to recombinant human IL17F. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL17F
Entrez GeneID
112744Gene Name
IL17F
Gene Alias
IL-17F, ML-1, ML1
Gene Description
interleukin 17F
Omim ID
606496Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq
Other Designations
cytokine ML-1
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Interactome
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Disease
- Arthritis
- Asthma
- Behcet Syndrome
- Cardiovascular Diseases
- Colitis
- Crohn Disease
- Dermatitis
- Diabetes Mellitus
- Dyspepsia
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Publication Reference
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Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production.
Starnes T, Robertson MJ, Sledge G, Kelich S, Nakshatri H, Broxmeyer HE, Hromas R.
Journal of Immunology 2001 Oct; 167(8):4137.
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Identification of a novel cytokine, ML-1, and its expression in subjects with asthma.
Kawaguchi M, Onuchic LF, Li XD, Essayan DM, Schroeder J, Xiao HQ, Liu MC, Krishnaswamy G, Germino G, Huang SK.
Journal of Immunology 2001 Oct; 167(8):4430.
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IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding.
Hymowitz SG, Filvaroff EH, Yin JP, Lee J, Cai L, Risser P, Maruoka M, Mao W, Foster J, Kelley RF, Pan G, Gurney AL, de Vos AM, Starovasnik MA.
The EMBO Journal 2001 Oct; 20(19):5332.
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Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production.
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