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Last updated: 2017/2/19

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VEGFA polyclonal antibody

  • Catalog # : PAB16916
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant VEGFA.
  • Immunogen:
  • Recombinant protein corresponding to 164 amino acids of bovine VEGFA.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Western Blot (1:333)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using VEGFA polyclonal antibody (Cat # PAB16916) shows detection of recombinant bovine VEGFA at 17-19.2 KDa (arrowhead). Approximately 2 ug of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA diluted 1 : 10. The primary antibody was used at a 1 : 333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • VEGFA
  • Gene Alias:
  • VEGF
  • Gene Description:
  • vascular endothelial growth factor A
  • Other Designations:
  • -
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