IL13 polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Western blot using IL13 polyclonal antibody (Cat # PAB16913) shows detection of recombinant swine IL13 at 13.2 KDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with IL13 polyclonal antibody. After washing, membrane was probed with Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL13.
Immunogen
Recombinant protein corresponding to full length swine IL13.
Host
Rabbit
Reactivity
Bovine, Pig, Sheep
Form
Lyophilized
Recommend Usage
ELISA (1:11000)
Western Blot (1.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using IL13 polyclonal antibody (Cat # PAB16913) shows detection of recombinant swine IL13 at 13.2 KDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with IL13 polyclonal antibody. After washing, membrane was probed with Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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PEBP1 Acts as a Rheostat Between Prosurvival Autophagy and Ferroptotic Death in Asthmatic Epithelial Cells.
Jinming Zhao, Haider H Dar, Yanhan Deng, Claudette M St Croix, Zhipeng Li, Yoshinori Minami, Indira H Shrivastava, Yulia Y Tyurina, Emily Etling, Joel C Rosenbaum, Tadao Nagasaki, John B Trudeau, Simon C Watkins, Ivet Bahar, Hülya Bayır, Andy P VanDemark, Valerian E Kagan, Sally E Wenzel.
PNAS 2020 Jun; 117(25):14376.
Application：WB-Ce, WB-Tr, Human, Human airway epithelial cells.
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Functional implications for signaling via the IL4R/IL13R complex on bovine cells.
Trigona WL, Brown WC, Estes DM.
Veterinary Immunology and Immunopathology 1999 Dec; 72(1-2):73.
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PEBP1 Acts as a Rheostat Between Prosurvival Autophagy and Ferroptotic Death in Asthmatic Epithelial Cells.
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