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Last updated: 2017/5/21

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Gdf15 polyclonal antibody

  • Catalog # : PAB16912
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Gdf15 .
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of mouse Gdf15 .
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:100000-1:120000)
    Western Blot (1:3000-1:7000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using Gdf15 polyclonal antibody (Cat # PAB16912).
    Lane 1, recombinant MBP-Gdf15 fusion protein (60 KDa) purified from E.coli.
    Lane 2, yeast cell lysate expressing SUMO-mouse Gdf15 (42 KDa).
    Lane 3, R&D human Gdf15 monomer purified from CHO-K1 cells (14 KDa).
    All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Gdf15 was detected using a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™4000MP Imaging System.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Gene Name:
  • Gdf15
  • Gene Alias:
  • MIC-1,NAG-1,SBF
  • Gene Description:
  • growth differentiation factor 15
  • Other Designations:
  • Growth/differentiation factor 15 precursor (GDF-15),OTTMUSP00000027988,OTTMUSP00000027989,macrophage inhibiting cytokine-1
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