Gdf15 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot using Gdf15 polyclonal antibody (Cat # PAB16912).
Lane 1, recombinant MBP-Gdf15 fusion protein (60 KDa) purified from E.coli.
Lane 2, yeast cell lysate expressing SUMO-mouse Gdf15 (42 KDa).
Lane 3, R&D human Gdf15 monomer purified from CHO-K1 cells (14 KDa).
All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Gdf15 was detected using a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™4000MP Imaging System.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Gdf15.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Gdf15 .
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
ELISA (1:100000-1:120000)
Western Blot (1:3000-1:7000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using Gdf15 polyclonal antibody (Cat # PAB16912).
Lane 1, recombinant MBP-Gdf15 fusion protein (60 KDa) purified from E.coli.
Lane 2, yeast cell lysate expressing SUMO-mouse Gdf15 (42 KDa).
Lane 3, R&D human Gdf15 monomer purified from CHO-K1 cells (14 KDa).
All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. Gdf15 was detected using a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™4000MP Imaging System.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Gdf15
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Publication Reference
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Changes in gene expression contribute to cancer prevention by COX inhibitors.
Baek SJ, Eling TE.
Progress in Lipid Research 2005 Nov; 45(1):1.
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H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.
Lindmark F, Zheng SL, Wiklund F, Bensen J, Balter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Gronberg H, Xu J.
Journal of the National Cancer Institute 2004 Aug; 96(16):1248.
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Changes in gene expression contribute to cancer prevention by COX inhibitors.
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