RRM2B polyclonal antibody
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More Files
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Western Blot
Western blot using RRM2B polyclonal antibody (Cat # PAB16911) shows detection of recombinant (Lanes 1 and 3) and endogenous protein (Lanes 1 to 4) in whole cell extracts from transfected 293T.
Lane 1 contains purified recombinant human p53R2.
Lane 2 contains 293T cells transfected with control vector.
Lane 3 contains 293T transfected with p53R2-myc.
Lane 4 : 293T transfected with ScRNA.
Lane 5 : 293T tranfected with p53R2 SiRNA The band at 40.7 KDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B.
The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9 KDa. The highest band at 44.3 KDa corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4°C. ECL detection was used.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RRM2B.
Immunogen
A synthetic peptide corresponding to N-terminus of human RRM2B .
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:3000)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using RRM2B polyclonal antibody (Cat # PAB16911) shows detection of recombinant (Lanes 1 and 3) and endogenous protein (Lanes 1 to 4) in whole cell extracts from transfected 293T.
Lane 1 contains purified recombinant human p53R2.
Lane 2 contains 293T cells transfected with control vector.
Lane 3 contains 293T transfected with p53R2-myc.
Lane 4 : 293T transfected with ScRNA.
Lane 5 : 293T tranfected with p53R2 SiRNA The band at 40.7 KDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B.
The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9 KDa. The highest band at 44.3 KDa corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4°C. ECL detection was used.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RRM2B
Entrez GeneID
50484Gene Name
RRM2B
Gene Alias
DKFZp686M05248, MGC102856, MGC42116, p53R2
Gene Description
ribonucleotide reductase M2 B (TP53 inducible)
Omim ID
604712Gene Ontology
HyperlinkOther Designations
p53-inducible ribonucleotide reductase small subunit 2 homolog|p53-inducible ribonucleotide reductase small subunit 2 long form|p53-inducible ribonucleotide reductase small subunit 2 short form gamma
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Interactome
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Pathway
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Disease
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Publication Reference
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PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
Lawlor MA, Alessi DR.
Journal of Cell Science 2001 Aug; 114(Pt 16):2903.
Application：IHC, WB-Tr, Human, Cancers, Mammalian cells.
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PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
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