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Last updated: 2017/10/15
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RRM2B polyclonal antibody

  • Catalog # : PAB16911
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RRM2B .
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human RRM2B .
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:3000)
    Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using RRM2B polyclonal antibody (Cat # PAB16911) shows detection of recombinant (Lanes 1 and 3) and endogenous protein (Lanes 1 to 4) in whole cell extracts from transfected 293T.
    Lane 1 contains purified recombinant human p53R2.
    Lane 2 contains 293T cells transfected with control vector.
    Lane 3 contains 293T transfected with p53R2-myc.
    Lane 4 : 293T transfected with ScRNA.
    Lane 5 : 293T tranfected with p53R2 SiRNA The band at 40.7 KDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B.
    The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9 KDa. The highest band at 44.3 KDa corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4°C. ECL detection was used.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Gene Name:
  • RRM2B
  • Gene Alias:
  • DKFZp686M05248,MGC102856,MGC42116,p53R2
  • Gene Description:
  • ribonucleotide reductase M2 B (TP53 inducible)
  • Other Designations:
  • p53-inducible ribonucleotide reductase small subunit 2 homolog,p53-inducible ribonucleotide reductase small subunit 2 long form,p53-inducible ribonucleotide reductase small subunit 2 short form gamma
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