Immunoblot using CASZ1 polyclonal antibody (Cat # PAB16910) diluted to 1 : 1,000 fold. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Lane 1, BE2 (s) cell lysate. Lane 2, BE2 (N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kDa). Lane 4, SY5Y transfected with hCASZ11 (190kDa). Secondary antibody using HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Personal communication, Carol Thiele, NCI, Bethesda, MD.