UBE2J2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using UBE2J2 polyclonal antibody (Cat # PAB16908) shows detection of UBE2J2 in 293 cells over-expressing Myc-UBE2J2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2J2.
Immunogen
A synthetic peptide corresponding to internal region of human UBE2J2 .
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot using UBE2J2 polyclonal antibody (Cat # PAB16908) shows detection of UBE2J2 in 293 cells over-expressing Myc-UBE2J2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — UBE2J2
Entrez GeneID
118424Gene Name
UBE2J2
Gene Alias
NCUBE2, PRO2121
Gene Description
ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
OTTHUMP00000001907|OTTHUMP00000002078|ubiquitin conjugating enzyme 6|ubiquitin conjugating enzyme E2, J2|yeast UBC6 homolog
-
Interactome
-
Pathway
-
Publication Reference
-
A role for mammalian Ubc6 homologues in ER-associated protein degradation.
Lenk U, Yu H, Walter J, Gelman MS, Hartmann E, Kopito RR, Sommer T.
Journal of Cell Science 2002 Jul; 115(Pt 14):3007.
-
Ubiquitin and its kin: how close are the family ties?
Jentsch S, Pyrowolakis G.
Trends in Cell Biology 2000 Aug; 10(8):335.
-
Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6.
Lester D, Farquharson C, Russell G, Houston B.
Biochemical and Biophysical Research Communications 2000 Mar; 269(2):474.
-
A role for mammalian Ubc6 homologues in ER-associated protein degradation.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com