Western blot using RPS6KA1 polyclonal antibody (Cat # PAB16907) shows detection of a band ~90 KDa in size corresponding to RPS6KA1 (arrowhead) in EGF stimulated (Lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (Lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1 : 1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
OTTHUMP00000004113,S6K-alpha 1,dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1),p90-RSK 1,ribosomal protein S6 kinase alpha 1,ribosomal protein S6 kinase, 90kD, 1,ribosomal protein S6 kinase, 90kD, polypeptide 1