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Last updated: 2016/12/4
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RPS6KA1 polyclonal antibody

  • Catalog # : PAB16907
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA1 .
  • Immunogen:
  • A synthetic peptide corresponding to a region near C-terminus end of human RPS6KA1 .
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Horse, Human, Mouse, Rat
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using RPS6KA1 polyclonal antibody (Cat # PAB16907) shows detection of a band ~90 KDa in size corresponding to RPS6KA1 (arrowhead) in EGF stimulated (Lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (Lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1 : 1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.
    Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6195
  • Gene Name:
  • RPS6KA1
  • Gene Alias:
  • HU-1,MAPKAPK1A,RSK,RSK1
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 1
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000004113,S6K-alpha 1,dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1),p90-RSK 1,ribosomal protein S6 kinase alpha 1,ribosomal protein S6 kinase, 90kD, 1,ribosomal protein S6 kinase, 90kD, polypeptide 1
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