RPS6KA1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using RPS6KA1 polyclonal antibody (Cat # PAB16907) shows detection of a band ~90 KDa in size corresponding to RPS6KA1 (arrowhead) in EGF stimulated (Lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (Lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1 : 1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.
Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA1.
Immunogen
A synthetic peptide corresponding to a region near C-terminus end of human RPS6KA1 .
Host
Rabbit
Reactivity
Bovine, Dog, Horse, Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using RPS6KA1 polyclonal antibody (Cat # PAB16907) shows detection of a band ~90 KDa in size corresponding to RPS6KA1 (arrowhead) in EGF stimulated (Lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (Lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1 : 1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.
Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6KA1
Entrez GeneID
6195Gene Name
RPS6KA1
Gene Alias
HU-1, MAPKAPK1A, RSK, RSK1
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 1
Omim ID
601684Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000004113|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|ribosomal protein S6 kinase alpha 1|ribosomal protein S6 kinase, 90kD, 1|ribosomal protein S6 kinase, 90kD, polypeptide 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase.
Chaturvedi D, Poppleton HM, Stringfield T, Barbier A, Patel TB.
Molecular and Cellular Biology 2006 Jun; 26(12):4586.
Application：WB, Human, B82L, HeLa cells.
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Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase.
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