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Last updated: 2017/6/18
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TRAF2 polyclonal antibody

  • Catalog # : PAB16905
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of TRAF2 .
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human TRAF2 .
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:60000-1:250000)
    Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using TRAF2 polyclonal antibody (Cat # PAB16905) shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 1 shows endogenous TRAF2 detected with antibody at 47 KDa (arrowhead). Lane 2 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 1 is unknown. Briefly, each lane contains approximately 14 ug of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1 : 500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1 : 20,000 dilution conjugated Gt-a-Rabbit DyLight 649 for 1 hr at room temperature. Fluorescence image was captured using the VersaDoc®Imaging System developed by Bio-Rad.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7186
  • Gene Name:
  • TRAF2
  • Gene Alias:
  • MGC:45012,TRAP,TRAP3
  • Gene Description:
  • TNF receptor-associated factor 2
  • Gene Summary:
  • The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022625,OTTHUMP00000064745,tumor necrosis factor type 2 receptor associated protein 3
  • Interactome
  • Interactome
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