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Last updated: 2017/8/13
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SAE1 polyclonal antibody

  • Catalog # : PAB16904
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SAE1.
  • Immunogen:
  • A synthetic peptide corresponding to a region surrounding S185 of human SAE1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using SAE1 polyclonal antibody (Cat # PAB16904) shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1 : 1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.
    Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • SAE1
  • Gene Alias:
  • AOS1,FLJ3091,HSPC140,SUA1
  • Gene Description:
  • SUMO1 activating enzyme subunit 1
  • Other Designations:
  • SUMO-1 activating enzyme E1 N subunit,SUMO-1 activating enzyme subunit 1,activator of SUMO1,sentrin/SUMO-activating protein AOS1,ubiquitin-like protein SUMO-1 activating enzyme
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