SAE1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using SAE1 polyclonal antibody (Cat # PAB16904) shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1 : 1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.
Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SAE1.
Immunogen
A synthetic peptide corresponding to a region surrounding S185 of human SAE1.
Host
Rabbit
Reactivity
Bovine, Dog, Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using SAE1 polyclonal antibody (Cat # PAB16904) shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1 : 1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.
Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SAE1
Entrez GeneID
10055Gene Name
SAE1
Gene Alias
AOS1, FLJ3091, HSPC140, SUA1
Gene Description
SUMO1 activating enzyme subunit 1
Gene Ontology
HyperlinkOther Designations
SUMO-1 activating enzyme E1 N subunit|SUMO-1 activating enzyme subunit 1|activator of SUMO1|sentrin/SUMO-activating protein AOS1|ubiquitin-like protein SUMO-1 activating enzyme
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Interactome
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Pathway
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Publication Reference
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Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1.
Lois LM, Lima CD.
The EMBO Journal 2005 Feb; 24(3):439.
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Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
Desterro JM, Rodriguez MS, Kemp GD, Hay RT.
The Journal of Biological Chemistry 1999 Apr; 274(15):10618.
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Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex.
Gong L, Li B, Millas S, Yeh ET.
FEBS Letters 1999 Apr; 448(1):185.
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Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1.
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