CENPQ polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using CENPQ polyclonal antibody (Cat # PAB16897) shows detection of endogenous CENPQ in a HeLa whole cell lysate (Lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5 ug/mL , followed by washes and incubation with to the secondary antibody.
Lane 1 : Lysates from HeLa cells transfected with control sh-virus.
Lane 2 : Lysates from HeLa cells transfected with CENPQ sh-virus.
Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CENPQ.
Immunogen
Recombinant protein corresponding to full length human CENPQ.
Host
Rabbit
Reactivity
Human, Macaque
Form
Liquid
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using CENPQ polyclonal antibody (Cat # PAB16897) shows detection of endogenous CENPQ in a HeLa whole cell lysate (Lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5 ug/mL , followed by washes and incubation with to the secondary antibody.
Lane 1 : Lysates from HeLa cells transfected with control sh-virus.
Lane 2 : Lysates from HeLa cells transfected with CENPQ sh-virus.
Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CENPQ
Entrez GeneID
55166Gene Name
CENPQ
Gene Alias
C6orf139, CENP-Q, FLJ10545
Gene Description
centromere protein Q
Omim ID
611506Gene Ontology
HyperlinkGene Summary
CENPQ is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM
Other Designations
OTTHUMP00000016579
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Interactome
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Publication Reference
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CENP-O class proteins form a stable complex and are required for proper kinetochore function.
Hori T, Okada M, Maenaka K, Fukagawa T.
Molecular Biology of the Cell 2008 Mar; 19(3):843.
Application：IF, WB, Chicken, DT40 cells.
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The human CENP-A centromeric nucleosome-associated complex.
Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW.
Nature Cell Biology 2006 May; 8(5):458.
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The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.
Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR 3rd, Desai A, Fukagawa T.
Nature Cell Biology 2006 May; 8(5):446.
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CENP-O class proteins form a stable complex and are required for proper kinetochore function.
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