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CENPQ polyclonal antibody

  • Catalog # : PAB16897
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant CENPQ.
  • Immunogen:
  • Recombinant protein corresponding to full length human CENPQ.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Macaque
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using CENPQ polyclonal antibody (Cat # PAB16897) shows detection of endogenous CENPQ in a HeLa whole cell lysate (Lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5 ug/mL , followed by washes and incubation with to the secondary antibody.
    Lane 1 : Lysates from HeLa cells transfected with control sh-virus.
    Lane 2 : Lysates from HeLa cells transfected with CENPQ sh-virus.
    Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • CENPQ
  • Gene Alias:
  • C6orf139,CENP-Q,FLJ10545
  • Gene Description:
  • centromere protein Q
  • Gene Summary:
  • CENPQ is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000016579
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