RGS21 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RGS21.
Immunogen
A synthetic peptide corresponding to internal region 16 amino acids of human RGS21.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RGS21 in HepG2 cell lysate with RGS21 polyclonal antibody (Cat # PAB16757) at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunohistochemistry
Immunocytochemistry
Immunocytochemistry of RGS21 in HepG2 cells with RGS21 polyclonal antibody (Cat # PAB16757) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — RGS21
Entrez GeneID
431704Protein Accession#
NP_001034241Gene Name
RGS21
Gene Alias
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Gene Description
regulator of G-protein signaling 21
Gene Ontology
HyperlinkGene Summary
Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins are GTPase-activating proteins for Gi (see GNAI1; MIM 139310) and Gq (see GNAQ; MIM 600998) class G-alpha proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination.[supplied by OMIM
Other Designations
regulator of G-protein signalling 21
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Publication Reference
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Isolation and expression pattern of RGS21 gene, a novel RGS member.
Li X, Chen L, Ji C, Liu B, Gu J, Xu J, Zou X, Gu S, Mao Y.
Acta Biochimica Polonica 2005 Sep; 52(4):943.
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RGS21 is a novel regulator of G protein signalling selectively expressed in subpopulations of taste bud cells.
von Buchholtz L, Elischer A, Tareilus E, Gouka R, Kaiser C, Breer H, Conzelmann S.
The European Journal of Neuroscience 2004 Mar; 19(6):1535.
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GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunits.
Berman DM, Wilkie TM, Gilman AG.
Cell 1996 Aug; 86(3):445.
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Isolation and expression pattern of RGS21 gene, a novel RGS member.
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