MC4R polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MC4R.
Immunogen
A synthetic peptide corresponding to N-terminus 19 amino acids of human MC4R.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of MC4R in rat brain tissue lysate with MC4R polyclonal antibody (Cat # PAB16756) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunohistochemistry
Immunohistochemistry of MC4R in human brain tissue with MC4R polyclonal antibody (Cat # PAB16756) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — MC4R
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Interactome
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Pathway
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Disease
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Publication Reference
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Melanocortin-4 receptor activation inhibits c-Jun N-terminal kinase activity and promotes insulin signaling.
Chai B, Li JY, Zhang W, Wang H, Mulholland MW.
Peptides 2009 Mar; 30(6):1098.
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Anatomy and regulation of the central melanocortin system.
Cone RD.
Nature Neuroscience 2005 May; 8(5):571.
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Activation of MAP kinase by MC4-R through PI3 kinase.
Vongs A, Lynn NM, Rosenblum CI.
Regulatory Peptides 2004 Aug; 120(1-3):113.
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Melanocortin-4 receptor activation inhibits c-Jun N-terminal kinase activity and promotes insulin signaling.
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