GLE1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of GLE1 in mouse brain tissue lysate with GLE1 polyclonal antibody (Cat # PAB16679) at (A) 1 and (B) 2 ug/mL .
Immunohistochemistry
Immunohistochemistry of GLE1 in mouse brain tissue with GLE1 polyclonal antibody (Cat # PAB16679) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GLE1.
Immunogen
A synthetic peptide corresponding to C-terminus 15 amino acids of human GLE1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of GLE1 in mouse brain tissue lysate with GLE1 polyclonal antibody (Cat # PAB16679) at (A) 1 and (B) 2 ug/mL .Immunohistochemistry
Immunohistochemistry of GLE1 in mouse brain tissue with GLE1 polyclonal antibody (Cat # PAB16679) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — GLE1
Entrez GeneID
2733Protein Accession#
NP_001003722Gene Name
GLE1
Gene Alias
GLE1L, LCCS, LCCS1, hGLE1
Gene Description
GLE1 RNA export mediator homolog (yeast)
Omim ID
603371Gene Ontology
HyperlinkGene Summary
This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
GLE1 RNA export mediator homolog|GLE1 RNA export mediator-like|GLE1-like, RNA export mediator|OTTHUMP00000022280|OTTHUMP00000022281
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Interactome
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Disease
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Publication Reference
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Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA.
Kendirgi F, Rexer DJ, Alcazar-Roman AR, Onishko HM, Wente SR.
Molecular Biology of the Cell 2005 Sep; 16(9):4304.
Application：WB, Human, HeLa cells, Recombinant protein.
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The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155.
Rayala HJ, Kendirgi F, Barry DM, Majerus PW, Wente SR.
Molecular & Cellular Proteomics 2004 Feb; 3(2):145.
Application：IF, WB, Human, HeLa cells.
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The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export.
Watkins JL, Murphy R, Emtage JL, Wente SR.
PNAS 1998 Jun; 95(12):6779.
Application：IF, WB-Ce, Human, HeLa cells..
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Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA.
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