htb1 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Identification of Histone H2B in crude extract of fussion yeast S. pombe with Histone H2B polyclonal antibody (Cat # PAB16672). The 17 kDa and 24~25 kDa band corresponds to the unmodified and the mono-ubiquitinated Histone H2B, respectively.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of histone H2B.
Immunogen
A synthetic peptide corresponding to N-terminus of Schizosaccharomyces pombe histone H2B.
Sequence
SAAEKKPASKAPAGKA
Host
Rabbit
Reactivity
S. pombe
Form
Liquid
Recommend Usage
Immunoprecipitation (CHIP assay)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Recombinant protein)
Identification of Histone H2B in crude extract of fussion yeast S. pombe with Histone H2B polyclonal antibody (Cat # PAB16672). The 17 kDa and 24~25 kDa band corresponds to the unmodified and the mono-ubiquitinated Histone H2B, respectively. -
Gene Info — htb1
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Publication Reference
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Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation.
Maruyama T, Nakamura T, Hayashi T, Yanagida M.
The EMBO Journal 2006 Jun; 25(11):2420.
Application：ChIP, WB, Yeast, Schizosaccharomyces pombe.
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Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation.
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