RANBP9 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant RANBP9.
Immunogen
Recombinant protein corresponding to the region from F133 to Y229 of human RANBP9.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blottinbg of crude extracts of HeLa (Lane 1) and U-2 OS (Lane 2) cells with RANBP9 polyclonal antibody (Cat # PAB16671).Immunocytochemistry
Immunoprecipitation
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Gene Info — RANBP9
Entrez GeneID
10048Gene Name
RANBP9
Gene Alias
RANBPM
Gene Description
RAN binding protein 9
Omim ID
603854Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq
Other Designations
OTTHUMP00000017269|novel centrosomal protein RanBPM|ran binding protein, centrosomal
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Interactome
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Publication Reference
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Multisite phosphorylation dictates selective E2-E3 pairing as revealed by Ubc8/UBE2H-GID/CTLH assemblies.
Jakub Chrustowicz, Dawafuti Sherpa, Jerry Li, Christine R Langlois, Eleftheria C Papadopoulou, D Tung Vu, Laura A Hehl, Özge Karayel, Viola Beier, Susanne von Gronau, Judith Müller, J Rajan Prabu, Matthias Mann, Gary Kleiger, Arno F Alpi, Brenda A Schulman.
Molecular Cell 2024 Jan; 84(2):293.
Application:WB, Human, HEK293T.
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GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme.
Dawafuti Sherpa, Jakub Chrustowicz, Shuai Qiao, Christine R Langlois, Laura A Hehl, Karthik Varma Gottemukkala, Fynn M Hansen, Ozge Karayel, Susanne von Gronau, J Rajan Prabu, Matthias Mann, Arno F Alpi, Brenda A Schulman.
Molecular Cell 2021 Jun; 81(11):2445.
Application:WB-Ce, Human, K-562 cells.
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A novel nuclear protein, Twa1, and Muskelin comprise a complex with RanBPM.
Umeda M, Nishitani H, Nishimoto T.
Gene 2003 Jan; 303:47.
Application:IF, IP, WB-Tr, Monkey, COS-7 cells.
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Full-sized RanBPM cDNA encodes a protein possessing a long stretch of proline and glutamine within the N-terminal region, comprising a large protein complex.
Nishitani H, Hirose E, Uchimura Y, Nakamura M, Umeda M, Nishii K, Mori N, Nishimoto T.
Gene 2001 Jul; 272(1-2):25.
Application:WB-Ce, WB-Tr, Dog, Human, Monkey, Mouse, HeLa, KB, Cos-7, BHK21, CHO, WEHI, FM3A cells.
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Multisite phosphorylation dictates selective E2-E3 pairing as revealed by Ubc8/UBE2H-GID/CTLH assemblies.
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