AFM polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AFM.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human AFM.
Sequence
C-NKDDRPKDLSLRE
Host
Goat
Theoretical MW (kDa)
69
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AFM polyclonal antibody (Cat # PAB16637) (0.3 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AFM
Entrez GeneID
173Protein Accession#
NP_001124.1Gene Name
AFM
Gene Alias
ALB2, ALBA, ALF, MGC125338, MGC125339
Gene Description
afamin
Omim ID
104145Gene Ontology
HyperlinkGene Summary
This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq
Other Designations
alpha-albumin
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Interactome
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Publication Reference
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Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier.
Kratzer I, Bernhart E, Wintersperger A, Hammer A, Waltl S, Malle E, Sperk G, Wietzorrek G, Dieplinger H, Sattler W.
Journal of Neurochemistry 2009 Feb; 108(3):707.
Application:IF, IHC, WB-Ce, Pig, Porcine brain capillary endothelial cells, brain.
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Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier.
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