IREB2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IREB2.
Immunogen
A synthetic peptide corresponding to internal region of human IREB2.
Sequence
C-SIHYEGSEYKLSHGS
Host
Goat
Theoretical MW (kDa)
105
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
IREB2 polyclonal antibody (Cat # PAB16635) (0.3 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IREB2 polyclonal antibody (Cat # PAB16635)(3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — IREB2
Entrez GeneID
3658Protein Accession#
NP_004127.1Gene Name
IREB2
Gene Alias
ACO3, FLJ23381, IRP2, IRP2AD
Gene Description
iron-responsive element binding protein 2
Omim ID
147582Gene Ontology
HyperlinkOther Designations
iron regulatory protein 2
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Interactome
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Disease
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Publication Reference
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Excess capacity of the iron regulatory protein system.
Wang W, Di X, D'Agostino RB Jr, Torti SV, Torti FM.
The Journal of Biological Chemistry 2007 Aug; 282(34):24650.
Application:WB-Tr, Human, HeLa cells.
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Excess capacity of the iron regulatory protein system.
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