Phosphothreonine polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against phosphothreonine.
Immunogen
Phosphothreonine conjugated with KLH.
Host
Rabbit
Specificity
Recognizes proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine. Species independent of cross reactivity.
Form
Liquid
Recommend Usage
ELISA (0.5 ug/mL)
Western Blot (2 ug/mL)
Immunoprecipitation (10 ug/mg lysate)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.0 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain extract with Phosphothreonine polyclonal antibody (Cat # PAB16622).
Lane 1, vanadium-treated.
Lane 2, control.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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