Rabbit polyclonal antibody raised against recombinant IFNG.
Immunogen:
Recombinant protein corresponding to human IFNG.
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Form:
Lyophilized
Purification:
Protein G affinity chromatography
Isotype:
IgG
Recommend Usage:
ELISA (1-2 ug/mL) Immunohistochemistry (2-10 ug/mL) Western Blot (2-10 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from PBS, pH 7.4.
Storage Instruction:
The lyophilized antibody is stable for at least 1 year from date of receipt at -20°C. After reconstitution with 0.01 M PBS, pH 7.4 or other diluents, this antibody can be stored in working aliquots at 2°C- 8°C for one month, or at -20°C for six months without detectable loss of activity. Aliquot to avoid repeated freezing and thawing.
Interferon-gamma (IFNG), or type II interferon, is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNG in the immune system stems in part from its ability to inhibit viral replication directly, but most importantly derives from its immunostimulatory and immunomodulatory effects. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 (MIM 186940) and CD8 (see MIM 186910) cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops (Schoenborn and Wilson, 2007 [PubMed 17981204]).[supplied by OMIM