Strep tag II polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of (A) 5 and (B) 25 ng of Strep-tag II recombinant protein with Strep tag II polyclonal antibody (Cat # PAB16602) at 1 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of Strep-tag II.
Immunogen
A synthetic peptide corresponding to Strep tag II.
Sequence
srWSHPQFEKsh
Host
Rabbit
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of (A) 5 and (B) 25 ng of Strep-tag II recombinant protein with Strep tag II polyclonal antibody (Cat # PAB16602) at 1 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin.
Schmidt TG, Koepke J, Frank R, Skerra A.
Journal of Molecular Biology 1996 Feb; 255(5):753.
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The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.
Schmidt TG, Skerra A.
Protein Engineering 1993 Jan; 6(1):109.
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Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin.
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