Strep tag II polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of Strep-tag II.
Immunogen
A synthetic peptide corresponding to Strep tag II.
Sequence
gggWSHPQFEK
Host
Rabbit
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of 50 ng of Strep-tag II recombinant protein with Strep tag II polyclonal antibody (Cat # PAB16601) at 1 ug/mL in (A) the absence or (B) the presence of blocking peptide.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Atovaquone and Pibrentasvir Inhibit the SARS-CoV-2 Endoribonuclease and Restrict Infection In Vitro but Not In Vivo.
Troy von Beck, Luis Mena Hernandez, Hongyi Zhou, Katharine Floyd, Mehul S Suthar, Jeffrey Skolnick, Joshy Jacob.
Viruses 2023 Aug; 15(9):1841.
Application:WB, Human, HEK293T cells.
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Phosphorylation of threonine residues on Shc promotes ligand binding and mediates crosstalk between MAPK and Akt pathways in breast cancer cells.
Suen KM, Lin CC, Seiler C, George R, Poncet-Montange G, Biter AB, Ahmed Z, Arold ST, Ladbury JE.
The International Journal of Biochemistry & Cell Biology 2017 Dec; 94:89.
Application:WB, Human, HEK 293T cells.
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Interaction with Shc prevents aberrant Erk activation in the absence of extracellular stimuli.
Suen KM, Lin CC, George R, Melo FA, Biggs ER, Ahmed Z, Drake MN, Arur S, Arold ST, Ladbury JE.
Nature Structural & Molecular Biology 2013 May; 20(5):620.
Application:WB-Tr, Human, HEK 293T cells.
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Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin.
Schmidt TG, Koepke J, Frank R, Skerra A.
Journal of Molecular Biology 1996 Feb; 255(5):753.
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The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.
Schmidt TG, Skerra A.
Protein Engineering 1993 Jan; 6(1):109.
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Atovaquone and Pibrentasvir Inhibit the SARS-CoV-2 Endoribonuclease and Restrict Infection In Vitro but Not In Vivo.
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