PIGY polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of PIGY in human spleen tissue lysate with PIGY polyclonal antibody (Cat # PAB16600) at (A) 1 and (B) 2 ug/mL .
Immunohistochemistry
Immunohistochemistry of PIGY in human spleen tissue with PIGY polyclonal antibody (Cat # PAB16600) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIGY.
Immunogen
A synthetic peptide corresponding to internal region 16 amino acids of human PIGY.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PIGY in human spleen tissue lysate with PIGY polyclonal antibody (Cat # PAB16600) at (A) 1 and (B) 2 ug/mL .Immunohistochemistry
Immunohistochemistry of PIGY in human spleen tissue with PIGY polyclonal antibody (Cat # PAB16600) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIGY
Entrez GeneID
84992Protein Accession#
NP_116295Gene Name
PIGY
Gene Alias
MGC14156, PIG-Y
Gene Description
phosphatidylinositol glycan anchor biosynthesis, class Y
Omim ID
610662Gene Ontology
HyperlinkGene Summary
This gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function. [provided by RefSeq
Other Designations
phosphatidylinositol N-acetylglucosaminyltransferase subunit Y|phosphatidylinositol glycan, class Y
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Interactome
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Pathway
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Disease
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Publication Reference
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The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component.
Murakami Y, Siripanyaphinyo U, Hong Y, Tashima Y, Maeda Y, Kinoshita T.
Molecular Biology of the Cell 2005 Nov; 16(11):5236.
Application：WB-Tr, Human, HeLa cells.
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Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins.
Eisenhaber B, Maurer-Stroh S, Novatchkova M, Schneider G, Eisenhaber F.
Bioessays 2003 Apr; 25(4):367.
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Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2.
Watanabe R, Murakami Y, Marmor MD, Inoue N, Maeda Y, Hino J, Kangawa K, Julius M, Kinoshita T.
The EMBO Journal 2000 Aug; 19(16):4402.
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The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component.
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