MARCH8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 polyclonal antibody (Cat # PAB16567) at (A) 0.5 ug/mL and (B) 1 ug/mL .
Immunocytochemistry
Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 polyclonal antibody (Cat # PAB16567) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MARCH8.
Immunogen
A synthetic peptide corresponding to C-terminus 17 amino acids of human MARCH8.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MARCH8 in HeLa cell lysate with MARCH8 polyclonal antibody (Cat # PAB16567) at (A) 0.5 ug/mL and (B) 1 ug/mL .Immunocytochemistry
Immunocytochemistry of MARCH8 in HeLa cells with MARCH8 polyclonal antibody (Cat # PAB16567) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — MARCH8
Entrez GeneID
220972Protein Accession#
NP_659458Gene Name
MARCH8
Gene Alias
MARCH-VIII, MIR, RNF178, c-MIR
Gene Description
membrane-associated ring finger (C3HC4) 8
Gene Ontology
HyperlinkGene Summary
cellular modulator of immune recognition|cellular modulator of immune recognition|cellular modulator of immune recognition (c-MIR)|membrane-associated RING-CH protein VIII
Other Designations
OTTHUMP00000058921|c-mir, cellular modulator of immune recognition|cellular modulator of immune recognition|cellular modulator of immune recognition (c-MIR)|membrane-associated RING-CH protein VIII
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Interactome
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Disease
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Publication Reference
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A novel family of membrane-bound E3 ubiquitin ligases.
Ohmura-Hoshino M, Goto E, Matsuki Y, Aoki M, Mito M, Uematsu M, Hotta H, Ishido S.
Journal of Biochemistry 2006 Aug; 140(2):147.
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Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins.
Bartee E, Mansouri M, Hovey Nerenberg BT, Gouveia K, Fruh K.
Journal of Virology 2004 Feb; 78(3):1109.
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c-MIR, a human E3 ubiquitin ligase, is a functional homolog of herpesvirus proteins MIR1 and MIR2 and has similar activity.
Goto E, Ishido S, Sato Y, Ohgimoto S, Ohgimoto K, Nagano-Fujii M, Hotta H.
The Journal of Biological Chemistry 2003 Apr; 278(17):14657.
Application：IP, Human, HEK 293T cells.
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A novel family of membrane-bound E3 ubiquitin ligases.
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