SPRED3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of SPRED3 in human brain tissue lysate with SPRED3 polyclonal antibody (Cat # PAB16561) at (A) 2 and (B) 4 ug/mL .
Immunohistochemistry
Immunohistochemistry of SPRED3 in human brain tissue with SPRED3 polyclonal antibody (Cat # PAB16561) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SPRED3.
Immunogen
A synthetic peptide corresponding to internal region 16 amino acids of human SPRED3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of SPRED3 in human brain tissue lysate with SPRED3 polyclonal antibody (Cat # PAB16561) at (A) 2 and (B) 4 ug/mL .Immunohistochemistry
Immunohistochemistry of SPRED3 in human brain tissue with SPRED3 polyclonal antibody (Cat # PAB16561) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPRED3
Entrez GeneID
399473Protein Accession#
NP_001035987Gene Name
SPRED3
Gene Alias
Eve-3
Gene Description
sprouty-related, EVH1 domain containing 3
Omim ID
609293Gene Ontology
HyperlinkGene Summary
SPRED3 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM
Other Designations
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Disease
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Publication Reference
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Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation.
Kato R, Nonami A, Taketomi T, Wakioka T, Kuroiwa A, Matsuda Y, Yoshimura A.
Biochemical and Biophysical Research Communications 2003 Mar; 302(4):767.
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Inhibition of angiogenesis by a mouse sprouty protein.
Lee SH, Schloss DJ, Jarvis L, Krasnow MA, Swain JL.
The Journal of Biological Chemistry 2001 Feb; 276(6):4128.
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Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed.
Minowada G, Jarvis LA, Chi CL, Neubuser A, Sun X, Hacohen N, Krasnow MA, Martin GR.
Development 1999 Oct; 126(20):4465.
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Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation.
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