POLR3F polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of POLR3F in human brain tissue lysate with POLR3F polyclonal antibody (Cat # PAB16541) at (Lane 1) 0.5 and (B) 1 ug/mL .
Immunohistochemistry
Immunohistochemistry of POLR3F in human brain tissue with POLR3F polyclonal antibody (Cat # PAB16541) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POLR3F.
Immunogen
A synthetic peptide corresponding to N-terminus 21 amino acids of human POLR3F.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of POLR3F in human brain tissue lysate with POLR3F polyclonal antibody (Cat # PAB16541) at (Lane 1) 0.5 and (B) 1 ug/mL .Immunohistochemistry
Immunohistochemistry of POLR3F in human brain tissue with POLR3F polyclonal antibody (Cat # PAB16541) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — POLR3F
Entrez GeneID
10621Protein Accession#
CAC11110Gene Name
POLR3F
Gene Alias
MGC13517, RPC39, RPC6
Gene Description
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. [provided by RefSeq
Other Designations
DNA-directed RNA polymerase III 39 kDa polypeptide F|DNA-directed RNA polymerases III 39 kDa polypeptide|OTTHUMP00000030359|RNA polymerase III C39 subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of host proteins required for HIV infection through a functional genomic screen.
Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ.
Science 2008 Jan; 319(5865):921.
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Spatial organization of transcription by RNA polymerase III.
Haeusler RA, Engelke DR.
Nucleic Acids Research 2006 Sep; 34(17):4826.
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Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.
Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP, Hernandez N.
Molecular and Cellular Biology 2002 Nov; 22(22):8044.
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Identification of host proteins required for HIV infection through a functional genomic screen.
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