P2RX7 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
P2RX7 polyclonal antibody (Cat # PAB16250) staining (0.3 ug/mL) of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer) with (Lane 2) and without (Lane 1) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
P2RX7 polyclonal antibody (Cat # PAB16250) (3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of P2RX7.
Immunogen
A synthetic peptide corresponding to N-terminus of human P2RX7.
Sequence
YETNKVTRIQSMNY-C
Host
Goat
Theoretical MW (kDa)
68.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
P2RX7 polyclonal antibody (Cat # PAB16250) staining (0.3 ug/mL) of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer) with (Lane 2) and without (Lane 1) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
P2RX7 polyclonal antibody (Cat # PAB16250) (3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — P2RX7
Entrez GeneID
5027Protein Accession#
NP_002553.2Gene Name
P2RX7
Gene Alias
MGC20089, P2X7
Gene Description
purinergic receptor P2X, ligand-gated ion channel, 7
Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq
Other Designations
ATP receptor|P2X purinoceptor 7|P2X7 receptor|P2Z receptor|purinergic receptor P2X7
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Interactome
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Pathway
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Disease
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Publication Reference
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Activated microglia in ischemic stroke penumbra upregulate MCP-1 and CCR2 expression in response to lysophosphatidylcholine derived from adjacent neurons and astrocytes.
Inose Y, Kato Y, Kitagawa K, Uchiyama S, Shibata N.
Neuropathology 2015 Jun; 35(3):209.
Application：IHC-P, ICC, Human, Mouse, Cerebral cortex, BV-2 cells.
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The human cathelicidin LL-37 modulates the activities of the P2X7 receptor in a structure-dependent manner.
Tomasinsig L, Pizzirani C, Skerlavaj B, Pellegatti P, Gulinelli S, Tossi A, Di Virgilio F, Zanetti M.
The Journal of Biological Chemistry 2008 Nov; 283(45):30471.
Application：WB-Tr, Human, HEK 293 cells.
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Activated microglia in ischemic stroke penumbra upregulate MCP-1 and CCR2 expression in response to lysophosphatidylcholine derived from adjacent neurons and astrocytes.
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