CANX polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CANX polyclonal antibody (Cat # PAB16249) (0.3 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CANX polyclonal antibody (Cat # PAB16249) (3.8 ug/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CANX.
Immunogen
A synthetic peptide corresponding near N-terminus of human CANX.
Sequence
C-SKTPELNLDQFHDKT
Host
Goat
Theoretical MW (kDa)
67.6, 67.2
Reactivity
Human, Mouse
Specificity
Reported variants represent identical protein: NP_001019820.1, NP_001737.1
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CANX polyclonal antibody (Cat # PAB16249) (0.3 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CANX polyclonal antibody (Cat # PAB16249) (3.8 ug/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CANX
Entrez GeneID
821Protein Accession#
NP_001737.1Gene Name
CANX
Gene Alias
CNX, FLJ26570, IP90, P90
Gene Description
calnexin
Omim ID
114217Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
Other Designations
major histocompatibility complex class I antigen-binding protein p88
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Interactome
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Pathway
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Publication Reference
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Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2.
Rosser MF, Grove DE, Chen L, Cyr DM.
Molecular Biology of the Cell 2008 Nov; 19(11):4570.
Application：IP, Human, HEK 293 cells.
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Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2.
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