RPS27A polyclonal antibody
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using RPS27A polyclonal antibody (Cat # PAB15860).
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Specification
Product Description
Rabbit polyclonal antibody raised against RPS27A.
Immunogen
Gluteraldehyde conjugated with ubiquitin.
Host
Rabbit
Reactivity
Bovine, Chicken, Fruit fly, Human, Mouse, Nematoda
Specificity
This recognizes ubiquitinated inclusion bodies and both the mono- and polyubiquitin forms. This recognizes aggresomes.
Form
Liquid
Recommend Usage
Immunofluorescence (1:500-1:1000)
Immunohistochemistry (Frozen sections) (1:500-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using RPS27A polyclonal antibody (Cat # PAB15860).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14.
Yanagawa T, Funasaka T, Tsutsumi S, Hu H, Watanabe H, Raz A.
Cancer Research 2007 Sep; 67(18):8682.
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Protein composition of the intranuclear inclusions of FXTAS.
Iwahashi CK, Yasui DH, An HJ, Greco CM, Tassone F, Nannen K, Babineau B, Lebrilla CB, Hagerman RJ, Hagerman PJ.
Brain 2006 Jan; 129(Pt 1):256.
Application：IF, Human, Inclusions from frontal cortex of patients with Fragile X-associated tremor/ataxia syndrome.
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Induction of inclusion formation and disruption of lamin A/C structure by premutation CGG-repeat RNA in human cultured neural cells.
Arocena DG, Iwahashi CK, Won N, Beilina A, Ludwig AL, Tassone F, Schwartz PH, Hagerman PJ.
Human Molecular Genetics 2005 Dec; 14(23):3661.
Application：IF, Human, Human neurally derived SK-N-MC cells.
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Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14.
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