WIZ polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
WIZ polyclonal antibody (Cat # PAB15682) (0.3 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of WIZ.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human WIZ.
Sequence
C-RSYIQGGRPFTKKFR
Host
Goat
Theoretical MW (kDa)
85.4
Reactivity
Human
Specificity
Approx 75 KDa band observed in human brain (cerebellum) lysates (calculated MW of 85.4 KDa according to NP_067064.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
WIZ polyclonal antibody (Cat # PAB15682) (0.3 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WIZ
Entrez GeneID
58525Protein Accession#
NP_067064.2Gene Name
WIZ
Gene Alias
ZNF803
Gene Description
widely interspaced zinc finger motifs
Gene Ontology
HyperlinkOther Designations
WIZ zinc finger|widely-interspaced zinc finger motifs
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Interactome
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Publication Reference
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Protein lysine methyltransferase G9a acts on non-histone targets.
Rathert P, Dhayalan A, Murakami M, Zhang X, Tamas R, Jurkowska R, Komatsu Y, Shinkai Y, Cheng X, Jeltsch A.
Nature Chemical Biology 2008 Jun; 4(6):344.
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Protein lysine methyltransferase G9a acts on non-histone targets.
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