Goat polyclonal antibody raised aganist synthetic peptide of SULF2.
A synthetic peptide corresponding to amino acids at internal region of human SULF2.
Theoretical MW (kDa):
Approx 90 KDa band observed in human ovary lysates (calculated MW of 98.2 KDa according to NP_940998.1). An additional band of 50 KDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Antigen affinity purification
ELISA (1:8000) Western Blot (0.3-1 ug/mL) The optimal working dilution should be determined by the end user.
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
SULF2 polyclonal antibody (Cat # PAB15671) (0.3 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM