SH2D3A polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
SH2D3A polyclonal antibody (Cat # PAB15664) (0.1 ug/mL) staining of human tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of SH2D3A.
Immunogen
A synthetic peptide corresponding to human SH2D3A.
Sequence
C-APRAERFEKFQR
Host
Goat
Theoretical MW (kDa)
63.1
Reactivity
Human
Specificity
Approximately 60 KDa band observed in human Lung and Tonsil lysates (calculated MW of 63.1 KDa according to NP_005481.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SH2D3A polyclonal antibody (Cat # PAB15664) (0.1 ug/mL) staining of human tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SH2D3A
Entrez GeneID
10045Protein Accession#
NP_005481.2Gene Name
SH2D3A
Gene Alias
NSP1
Gene Description
SH2 domain containing 3A
Omim ID
604721Gene Ontology
HyperlinkOther Designations
SH2 domain-containing 3A|novel SH2-containing protein 1
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Interactome
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Publication Reference
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AND-34/BCAR3 differs from other NSP homologs in induction of anti-estrogen resistance, cyclin D1 promoter activation and altered breast cancer cell morphology.
Near RI, Zhang Y, Makkinje A, Vanden Borre P, Lerner A.
Journal of Cellular Physiology 2007 Sep; 212(3):655.
Application：WB-Tr, Human, HEK 293 cells.
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AND-34/BCAR3 differs from other NSP homologs in induction of anti-estrogen resistance, cyclin D1 promoter activation and altered breast cancer cell morphology.
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