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Last updated: 2017/8/20
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SFRP4 polyclonal antibody

  • Catalog # : PAB15663
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised aganist synthetic peptide of SFRP4.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human SFRP4.
  • Sequence:
  • TNSSCQCPHILPHQD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 39.8
  • Reactivity:
  • Human
  • Specificity:
  • Approximately 40 KDa band observed in human uterus lysates (calculated MW of 39.8 KDa according to NP_003005.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • SFRP4 polyclonal antibody (Cat # PAB15663) (0.3 ug/mL) staining of human uterus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • SFRP4 polyclonal antibody (Cat # PAB15663) (3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6424
  • Gene Name:
  • SFRP4
  • Gene Alias:
  • FRP-4,FRPHE,MGC26498
  • Gene Description:
  • secreted frizzled-related protein 4
  • Gene Summary:
  • Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq
  • Other Designations:
  • secreted frizzled-related protein 4,secreted frizzled-related protein 4; secreted frizzled-related protein 4
  • Gene Pathway
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