SFRP4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SFRP4 polyclonal antibody (Cat # PAB15663) (0.3 ug/mL) staining of human uterus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SFRP4 polyclonal antibody (Cat # PAB15663) (3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of SFRP4.
Immunogen
A synthetic peptide corresponding to internal region of human SFRP4.
Sequence
TNSSCQCPHILPHQD
Host
Goat
Theoretical MW (kDa)
39.8
Reactivity
Human
Specificity
Approximately 40 KDa band observed in human uterus lysates (calculated MW of 39.8 KDa according to NP_003005.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SFRP4 polyclonal antibody (Cat # PAB15663) (0.3 ug/mL) staining of human uterus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SFRP4 polyclonal antibody (Cat # PAB15663) (3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SFRP4
Entrez GeneID
6424Protein Accession#
NP_003005.2Gene Name
SFRP4
Gene Alias
FRP-4, FRPHE, MGC26498
Gene Description
secreted frizzled-related protein 4
Omim ID
606570Gene Ontology
HyperlinkGene Summary
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq
Other Designations
secreted frizzled-related protein 4|secreted frizzled-related protein 4; secreted frizzled-related protein 4
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Interactome
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Pathway
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Disease
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Publication Reference
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SFRPs Are Biphasic Modulators of Wnt-Signaling-Elicited Cancer Stem Cell Properties beyond Extracellular Control.
Liang CJ, Wang ZW, Chang YW, Lee KC, Lin WH, Lee JL.
Cell Reports 2019 Aug; 28(6):1511.
Application：WB, Human, H1299, H322, H358, H460, HM20 cells.
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Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R.
Genes & Development 2007 Dec; 21(23):3110.
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SFRPs Are Biphasic Modulators of Wnt-Signaling-Elicited Cancer Stem Cell Properties beyond Extracellular Control.
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