PALLD polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PALLD polyclonal antibody (Cat # PAB15653) (0.1 ug/mL) staining of human umbillical cord lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of PALLD.
Immunogen
A synthetic peptide corresponding to amino acids 895-907 of human PALLD.
Sequence
C-RDSGDENEPIQER
Host
Goat
Theoretical MW (kDa)
122
Reactivity
Human
Specificity
Approximately 125 KDa band observed in human umbillical cord lysates (calculated MW of 122 KDa according to NP_057165.3).
An additional band of 55 KDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PALLD polyclonal antibody (Cat # PAB15653) (0.1 ug/mL) staining of human umbillical cord lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PALLD
Entrez GeneID
23022Protein Accession#
NP_057165.3Gene Name
PALLD
Gene Alias
CGI-151, FLJ22190, FLJ38193, FLJ39139, KIAA0992, PNCA1, SIH002
Gene Description
palladin, cytoskeletal associated protein
Gene Ontology
HyperlinkGene Summary
Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction.[supplied by OMIM
Other Designations
palladin|sarcoma antigen NY-SAR-77
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Interactome
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Disease
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Publication Reference
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Five common gene variants identify elevated genetic risk for coronary heart disease.
Bare LA, Morrison AC, Rowland CM, Shiffman D, Luke MM, Iakoubova OA, Kane JP, Malloy MJ, Ellis SG, Pankow JS, Willerson JT, Devlin JJ, Boerwinkle E.
Genetics in Medicine 2007 Oct; 9(10):682.
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Five common gene variants identify elevated genetic risk for coronary heart disease.
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