CTAG1B polyclonal antibody
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More Files
- More Functions
Immunofluorescence
CTAG1B polyclonal antibody (Cat # PAB15650) Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
CTAG1B polyclonal antibody (Cat # PAB15650) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of CTAG1B.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human CTAG1B.
Sequence
C-AELARRSLAQDAP
Host
Goat
Theoretical MW (kDa)
18
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
Antibody Microarray
ELISA (1:16000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
CTAG1B polyclonal antibody (Cat # PAB15650) Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
CTAG1B polyclonal antibody (Cat # PAB15650) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — CTAG1B
Entrez GeneID
1485Protein Accession#
NP_001318.1Gene Name
CTAG1B
Gene Alias
CTAG, CTAG1, ESO1, LAGE-2, LAGE2B, NY-ESO-1
Gene Description
cancer/testis antigen 1B
Omim ID
300156Gene Ontology
HyperlinkGene Summary
Cancer/testis antigens, such as CTAG1B, are expressed in a variety of malignant tumors but soley in testis among normal adult tissues (Yoshida et al., 2006 [PubMed 16596224]).[supplied by OMIM
Other Designations
New York esophageal squamous cell carcinoma 1|OTTHUMP00000026025|OTTHUMP00000026042|cancer antigen 3
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Interactome
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Publication Reference
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Immunization of malignant melanoma patients with full-length NY-ESO-1 protein using TLR7 agonist imiquimod as vaccine adjuvant.
Adams S, O'Neill DW, Nonaka D, Hardin E, Chiriboga L, Siu K, Cruz CM, Angiulli A, Angiulli F, Ritter E, Holman RM, Shapiro RL, Berman RS, Berner N, Shao Y, Manches O, Pan L, Venhaus RR, Hoffman EW, Jungbluth A, Gnjatic S, Old L, Pavlick AC, Bhardwaj N.
Journal of Immunology 2008 Jul; 181(1):776.
Application：ELISA, Func, Human, Plasma from patients with malignant melanoma.
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Immunization of malignant melanoma patients with full-length NY-ESO-1 protein using TLR7 agonist imiquimod as vaccine adjuvant.
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