MC5R polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of MC5R.
Immunogen
A synthetic peptide corresponding to human MC5R.
Sequence
PNVKNKSSPCED
Host
Goat
Theoretical MW (kDa)
36.6
Reactivity
Human, Mouse
Specificity
Approximately 37 KDa band observed in human and mouse skeletal muscle lysates (calculated MW of 36.6 KDa according to NP_005904.1).
An additional band of unknown identity was also consistently observed at 30KDa. This band was successfully blocked by incubation with the immunizing peptide.Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MC5R polyclonal antibody (Cat # PAB15646) (0.3 ug/mL) staining of mouse muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MC5R
Entrez GeneID
4161Protein Accession#
NP_005904.1Gene Name
MC5R
Gene Alias
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Gene Description
melanocortin 5 receptor
Omim ID
600042Gene Ontology
HyperlinkOther Designations
melanocortin receptor 5
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Interactome
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Pathway
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Disease
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Publication Reference
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Further structure-activity studies of lactam derivatives of MT-II and SHU-9119: their activity and selectivity at human melanocortin receptors 3, 4, and 5.
Grieco P, Cai M, Han G, Trivedi D, Campiglia P, Novellino E, Hruby VJ.
Peptides 2007 Jun; 28(6):1191.
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Further structure-activity studies of lactam derivatives of MT-II and SHU-9119: their activity and selectivity at human melanocortin receptors 3, 4, and 5.
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