ITM2B polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ITM2B polyclonal antibody (Cat # PAB15636) (1 ug/mL) staining of human hippocampus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of ITM2B.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human ITM2B.
Sequence
C-HDKETYKLQRRETIK
Host
Goat
Theoretical MW (kDa)
30.3
Reactivity
Human
Specificity
Approximately 33 KDa band observed in human brain (cerebellum and hippocampus) lysates (calculated MW of 30.3 KDa according to NP_068839.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ITM2B polyclonal antibody (Cat # PAB15636) (1 ug/mL) staining of human hippocampus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ITM2B
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Interactome
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Publication Reference
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Understanding the development of human bladder cancer by using a whole-organ genomic mapping strategy.
Majewski T, Lee S, Jeong J, Yoon DS, Kram A, Kim MS, Tuziak T, Bondaruk J, Lee S, Park WS, Tang KS, Chung W, Shen L, Ahmed SS, Johnston DA, Grossman HB, Dinney CP, Zhou JH, Harris RA, Snyder C, Filipek S, Narod SA, Watson P, Lynch HT, Gazdar A, Bar-Eli M, Wu XF, McConkey DJ, Baggerly K, Issa JP, Benedict WF, Scherer SE, Czerniak B.
Laboratory Investigation; a Journal of Technical Methods and Pathology 2008 May; 88(7):694.
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Understanding the development of human bladder cancer by using a whole-organ genomic mapping strategy.
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