NUAK1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NUAK1 polyclonal antibody (Cat # PAB15608) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised aganist synthetic peptide of NUAK1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human NUAK1.
Sequence
C-QNRPRPQYLKRYRNR
Host
Goat
Theoretical MW (kDa)
74.3
Reactivity
Human
Specificity
Approximately 75 KDa band observed in human kidney, muscle and ovary lysates (calculated MW of 74.3 KDa according to NP_055655.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NUAK1 polyclonal antibody (Cat # PAB15608) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUAK1
Entrez GeneID
9891Protein Accession#
NP_055655.1Gene Name
NUAK1
Gene Alias
ARK5, KIAA0537
Gene Description
NUAK family, SNF1-like kinase, 1
Omim ID
608130Gene Ontology
HyperlinkGene Summary
SNF1-like kinase
Other Designations
AMP-activated protein kinase family member 5|AMPK-related protein kinase 5
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Interactome
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Disease
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Publication Reference
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Strong association of ARK5 with tumor invasion and metastasis.
Kusakai G, Suzuki A, Ogura T, Kaminishi M, Esumi H.
Journal of Experimental & Clinical Cancer Research 2004 Jun; 23(2):263.
Application：WB, Human, DLD-1, PANC-1 cells.
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Strong association of ARK5 with tumor invasion and metastasis.
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