Streptococcus NADase polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NADase.
Immunogen
Recombinant protein corresponding to hemolytic Streptococcus NADase.
Host
Rabbit
Specificity
This antibody reacts with NADase of Group A, C, and G origins.
Form
Liquid
Recommend Usage
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Detection of NADase in the culture supernatant of Hemolytic streptococci with NADase polyclonal antibody (Cat # PAB15599).
Lane 1 : Culture supernatant of group A streptococcus
Lane 2 : Culture supernatant of group C streptococcus
Lane 3 : Culture medium only (negative control)Immunoprecipitation
Inhibition Assay
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Publication Reference
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Biochemical and genetic properties of Paenibacillus glycosyl hydrolase having chitosanase activity and discoidin domain.
Kimoto H, Kusaoke H, Yamamoto I, Fujii Y, Onodera T, Taketo A.
The Journal of Biological Chemistry 2002 Apr; 277(17):14695.
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Molecular epidemiology of nga and NAD glycohydrolase/ADP-ribosyltransferase activity among Streptococcus pyogenes causing streptococcal toxic shock syndrome.
Stevens DL, Salmi DB, McIndoo ER, Bryant AE.
The Journal of Infectious Diseases 2000 Oct; 182(4):1117.
Application:WB, Human, Streptococcus pyogenes strains used for this study were clinical isolates obtained from patients with a variety of GAS infections.
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Biochemical and genetic properties of Paenibacillus glycosyl hydrolase having chitosanase activity and discoidin domain.
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