NOB1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of RPN7 (51.7 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN7 polyclonal antibody (Cat # PAB15596).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NOB1.
Immunogen
Recombinant protein corresponding to Saccharomyces cerevisiae NOB1.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody react with S. cerevisiae NOB1.
Form
Liquid
Recommend Usage
Western Blot (1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of RPN7 (51.7 kDa) in the crude extract of S. cerevisiae by Western blotting using RPN7 polyclonal antibody (Cat # PAB15596).Immunoprecipitation
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Gene Info — NOB1
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Publication Reference
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Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae.
Tone Y, Toh-E A.
Genes & Development 2002 Dec; 16(24):3142.
Application：WB-Ce, Yeast, S. cerevisiae J106, W303-1A, Y184 strains.
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The ubiquitin system.
Hershko A, Ciechanover A.
Annual Review of Biochemistry 1998 Jul; 67:425.
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Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae.
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